Tells you to do something, its probably for a good reason.
Like the fact that antibodies really do need to be at the correct pH. So when he says you need to check the pH BEFORE you start the blotting, its not just to make you do another step, its so that you both don't have the headache of checking it later.
Lesson learned. There is a reason (9 times out of 10) why you PI wants you to do something a very specific way.
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